Sequential injection extraction based on restricted access material for determination of furosemide in serum

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Huclova, J (Author) Other; Satinsky, D (Author) Other; Maia, T (Author) Other; Karlicek, R (Author) Other; Solich, P (Author) Other; Araujo, AN (Author) FFUP

Journal

Title: Journal of Chromatography A

Vol. 1087

Pages: 245-251

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 25 Citations

ISI Web of Science

Scopus - 25 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-000-1BA

DOI: 10.1016/j.chroma.2004.11.055

Resumo (PT): Restricted access material (RAM) column containing 25 μm C18 alkyl-diol support was integrated into the sequential injection analysis (SIA) manifold and the SIA–RAM system was tested for direct determination of furosemide in serum. LiChrospher® ADS column based on restricted access material is proposed to direct injection of biofluids. The integration of RAM material into SIA enabled creation of a comprehensive on-line sample clean-up technique combined with fluorescence quantitation of analyte. Centrifuged and diluted serum sample was aspirated into the system and loaded onto the column using acetonitrile–water (2:98), pH 2.7. The analyte was retained on the column while proteins contained in the sample were removed to the waste without precipitation and clogging the column. Interfering substances complicating the detection were washed out by acetonitrile–water (15:85), pH 2.7 in the next step. The extracted analyte was eluted by means of acetonitrile–water (25:75), pH 2.3 to the fluorescence detector (emission filter 385 nm). The whole procedure comprising sample pre-treatment, analyte detection and column reconditioning took 20 min. The recoveries of furosemide from serum lay between 101.4 and 103.4% for three concentrations of analyte. <br> <br> Keywords: Furosemide; Sequential injection analysis; Restricted access materials; Serum <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4F00TNR-2&_user=2460038&_coverDate=09%2F16%2F2005&_rdoc=33&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235248%232005%23989129998%23603067%23FLA%23display%23Volume)&_cdi=5248&_sort=d&_docanchor=&_ct=39&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=1c555b78cc2eeff359024989a2257f41 "> Texto integral </a> <br> <br>

Abstract (EN): Restricted access material (RAM) column containing 25 mu m C-18 alkyl-diol support was integrated into the sequential injection analysis (SIA) manifold and the SIA-RAM system was tested for direct determination of furosemide in serum. LiChrospher((R)) ADS column based on restricted access material is proposed to direct injection of biofluids. The integration of RAM material into SIA enabled creation of a comprehensive on-line sample clean-up technique combined with fluorescence quantitation of analyte. Centrifuged and diluted serum sample was aspirated into the system and loaded onto the column using acetonitrile-water (2:98), pH 2.7. The analyte was retained on the column while proteins contained in the sample were removed to the waste without precipitation and clogging the column. Interfering substances complicating the detection were washed out by acetonitrile-water (15:85), pH 2.7 in the next step. The extracted analyte was eluted by means of acetonitrile-water (25:75), pH 2.3 to the fluorescence detector (emission filter 385 nm). The whole procedure comprising sample pre-treatment, analyte detection and column reconditioning took 20 min. The recoveries of furosemide from serum lay between 101.4 and 103.4% for three concentrations of analyte.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7