Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Miguel Ferreira da Silva (Author) Other; Ivone Vaz Moreira (Author) FEUP; Maria Gonzalez Pajuelo (Author) Other; Olga C Nunes (Author) FEUP; Celia M Manaia (Author) Other

Journal

Title: FEMS Microbiology Ecology

Vol. 60 No. 1

Pages: 166-176

ISSN: 0168-6496

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 125 Citations

ISI Web of Science

Scopus - 177 Citations

COMPENDEX

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Natural sciences

Other information

Authenticus ID: P-004-AVS

DOI: 10.1111/j.1574-6941.2006.00268.x

Abstract (EN): Over 18 months, enterobacteria were isolated from the raw (189 isolates) and treated (156 isolates) wastewater of a municipal treatment plant. The isolates were identified as members of the genera Escherichia (76%), Shigella (7%), Klebsiella (12%) and Acinetobacter (4%). Antimicrobial susceptibility phenotypes were determined using the agar diffusion method for the antibiotics amoxicillin, gentamicin, ciprofloxacin, sulfamethoxazole/trimethoprim, tetracycline and cephalothin, the disinfectants hydrogen peroxide, sodium hypochlorite, quaternary ammonium/formaldehyde and iodine, and the heavy metals nickel, cadmium, chromium, mercury and zinc. Class 1 integrons were detected by PCR amplification using the primers CS5 and CS3. Compared with the raw influent, the treated wastewater presented higher relative proportions of Escherichia spp. isolates resistant to ciprofloxacin and cephalothin (P < 0.0001 and P < 0.05, respectively). Except for mercury, which showed a positive correlation with tetracycline and sulfamethoxazole/trimethoprim, no significant positive correlations were observed between antibiotic, disinfectant and heavy metal resistance. The variable regions of class 1 integrons, detected in c. 10% of the Escherichia spp. isolates, contained predominantly the gene cassettes aadA1/dhfrI.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11