Immobilization of commercial laccase on spent grain

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Andreia Machado da Silva (Author) Other; Ana P. M. Tavares (Author) FEUP; Cristina M. R. Rocha (Author) Other; Raquel O. Cristóvão (Author) FEUP; José A. Teixeira (Author) Other; Eugénia A. Macedo (Author) FEUP

Journal

Title: Process Biochemistry

Vol. 47 No. 7

Pages: 1095-1101

ISSN: 1359-5113

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 57 Citations

ISI Web of Science

Scopus - 63 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

LA 20 - Projeto Estratégico-LA 20 - 2011-2012

Other information

Authenticus ID: P-002-85Z

DOI: 10.1016/j.procbio.2012.03.021

Abstract (EN): aim of this work was to assess the possibility of using beer spent grain (a byproduct of beer's brewing industry) as a carrier for laccase immobilization. Both adsorption (on spent grain - SG and on digested spent grain - DSG)and covalent binding (using glycidol and glyciclol followed by ethylenediamine on DSG) were used. The effect of different immobilization conditions on the immobilization yields and recovered activities such as contact time, enzyme concentration and pH was evaluated. For the best conditions, immobilization yields, recovered activities and thermal, operational and storage stabilities were also evaluated. Finally, the Michaelis-Menten mechanism was applied and the parameter with respect to ABTS oxidation was determined. Enzyme immobilization on DSG led to the best enzyme activities (recovered activities as high as 90%) and to high storage and operational stabilities (10 cycles). Thermal stability was also improved and the half-life of immobilized laccase in SG increased from 0.64h to 1.1 h at 70 degrees C.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7