Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Vitorino, IR (Author) Other; Lobo-da-Cunha, A (Author) Other; Vitor Vasconcelos (Author) FCUP; lage, om (Author) FCUP

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 72

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 3 Citations

Scopus

Other information

Authenticus ID: P-00W-RSA

DOI: 10.1099/ijsem.0.005425

Abstract (EN): The phylum Planctomycetota is constituted by bacteria with unique features that are well adapted to a vast range of habitats. Here, we describe a novel planctomycete isolated from marine sediments collected on a beach in Matosinhos (Portugal) using an iChip-based culturing technique. Strain ICM_H10(T) forms beige-coloured colonies in modified M14 medium and its cells are spherical to ovoid in shape, stalked, rosette-forming and showing motility in a phase of the life cycle. Transmission electron microscopy observations showed a typical planctomycetal cell plan and cell division by budding. This strain requires salt for growth and grows in the range of 2.0-5.0% (w/v) NaCl, from 20 to 37 degrees C, within a pH of 6.0-9.0 and is able to use diverse nitrogen and carbon sources. It is heterotrophic, aerobic and capable of microaerobic growth. This strain has a genome size of approximately 6.0 Mb and a G+C content of 58.1 mol%. A 16S rRNA gene-based phylogenetic analysis supports the association of strain ICM_H10(T) to the phylum Planctomycetota and the family Planctomycetaceae, as it shares only 96.8 and 96.4% similarity to its closest relatives Rubinisphaera italica Pan54(T) and Rubinisphaera brasiliensis IFAM 1448(T), respectively. Other phylogenetic markers also support the separation of this strain into a novel species. Morphological, physiological and genomic comparisons between strain ICM_H10(T) and its closest relatives strongly suggest that ICM_H10T represents a new species of the genus Rubinisphaera, for which we propose the name Rubinisphaera margarita sp. nov., with ICM_H10(T) (=CECT 30326(T)=LMG 32234(T)) as type strain.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10