Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Vitorino, IR (Author) Other; Klimek, D (Author) Other; Calusinska, M (Author) Other; Lobo-da-Cunha, A (Author) Other; Vitor Vasconcelos (Author) FCUP; lage, om (Author) FCUP

Journal

Title: Microorganisms

Vol. 10

Final page: 2151

Publisher: MDPI

Indexing

ISI Web of Knowledge - 2 Citations

Scopus

Other information

Authenticus ID: P-00X-TJW

DOI: 10.3390/microorganisms10112151

Abstract (EN): The phylum Planctomycetota is known for having uncommon biological features. Recently, biotechnological applications of its members have started to be explored, namely in the genus Stieleria. Here, we formally describe a novel Stieleria isolate designated as strain ICT_E10.1(T), obtained from sediments collected in the Tagus estuary (Portugal). Strain ICT_E10.1(T) is pink-pigmented, spherical to ovoid in shape, and 1.7 mu m +/- 0.3 x 1.4 mu m +/- 0.3 in size. Cells cluster strongly in aggregates or small chains, divide by budding, and have prominent fimbriae. Strain ICT_E10.1(T) is heterotrophic and aerobic. Growth occurs from 20 to 30 degrees C, from 0.5 to 3% (w/v) NaCl, and from pH 6.5 to 11.0. The analysis of the 16S rRNA gene sequence placed strain ICT_E10.1(T) into the genus Stieleria with Stieleria neptunia Enr13(T) as the closest validly described relative. The genome size is 9,813,311 bp and the DNA G+C content is 58.8 mol%. Morphological, physiological, and genomic analyses support the separation of this strain into a novel species, for which we propose the name Stieleria sedimenti represented by strain ICT_E10.1(T) as the type of strain (=CECT 30514(T)= DSM 113784(T)). Furthermore, this isolate showed biotechnological potential by displaying relevant biosynthetic gene clusters and potent activity against Staphylococcus aureus.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18