Interference of chitosan in glucose analysis by high-performance liquid chromatography with evaporative light scattering detection

• Type: Article in International Scientific Journal
• Date: 2008

Authors

B. N. Estevinho (Author) Other; A. Ferraz (Author) Other; F. Rocha (Author) FEUP; A. Alves (Author) FEUP; L. Santos (Author) FEUP

Journal

Title: Analytical and Bioanalytical Chemistry

Vol. 391 No. 4

Pages: 1183-1188

ISSN: 1618-2642

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 8 Citations

ISI Web of Science

Scopus - 10 Citations

COMPENDEX

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-003-YMG

DOI: 10.1007/s00216-008-1832-3

Abstract (EN): The purpose of this work was to quantify glucose in aqueous solutions containing chitosan by high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD). Chitosan is a natural compound that is used alone or as an additive in several formulations. Microencapsulation of bioactive compounds such as glucose, by means of chitosan, is being explored, but difficulties arise when glucose needs to be determined in the presence of chitosan. HPLC is the technique most commonly used for glucose analysis, and ELSD may offer advantages (e.g. sensitivty and the possibility of operating in gradient mode) compared with other detectors. The influence of chitosan in the analysis of glucose by HPLC with ELSD was investigated at different pH values of the aqueous solutions. Isocratic elution with an acetonitrile/water mixture (80:20, v/v) and water washing between runs were the best options to avoid the mucoadhesive properties of chitosan, which are responsible for column degradation and variability of the retention time of glucose. The developed methodology was considered completely adequate for rapid glucose analysis in aqueous solutions with low pH (< 3), in the presence of chitosan.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6