Acinetobacter rudis sp nov., isolated from raw milk and raw wastewater

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Ivone Vaz Moreira (Author) FEUP; Ana Novo (Author) Other; Elionora Hantsis Zacharov (Author) Other; Ana Rita Lopes (Author) Other; Margarita Gomila (Author) Other; Olga C Nunes (Author) FEUP; Celia M Manaia (Author) Other; Malka Halpern (Author) Other

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 61 No. Part 9

Pages: 2837-2843

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 27 Citations

Scopus - 37 Citations

Scientific classification

CORDIS: Technological sciences > Engineering > Chemical engineering ; Natural sciences > Biological sciences > Biology > Microbiology

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-J0Y

DOI: 10.1099/ijs.0.027045-0

Abstract (EN): Two bacterial strains, G3O(T) and A1PC16, isolated respectively from raw milk and raw wastewater, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of these strains in the genus Acinetobacter, with Q-8 and Q-9 as the major respiratory quinones, genomic DNA G+C contents within the range observed for this genus (38-47 mol%) and C(16:0), C(18:1)omega 9c and C(16:1)omega 7c/iso-C(15:0) 2-OH as the predominant fatty acids. The observation of 16S rRNA gene sequence similarity lower than 97% with other Acinetobacter species with validly published names led to the hypothesis that these isolates could represent a novel species. This hypothesis was supported by comparative analysis of partial sequences of the genes rpoB and gyrB, which showed that strains G3O(T) and A1PC16 did not cluster with any species with validly published names, forming a distinct lineage. DNA DNA hybridizations confirmed that the two strains were members of the same species, which could be distinguished from their congeners by several phenotypic characteristics. On the basis of these arguments, it is proposed that strains G3O(T) and A1PC16 represent a novel species, for which the name Acinetobacter rudis sp. nov. is proposed. The type strain is strain G3O(T) (=LMG 26107(T) =CCUG 57889(T) =DSM 24031(T) =CECT 7818(T)).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7