Paenibacillus residui sp. nov., isolated from urban waste compost

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Ivone Vaz Moreira (Author) FEUP; Vania Figueira (Author) Other; Ana Rita Lopes (Author) Other; Ruediger Pukall (Author) Other; Cathrin Sproeer (Author) Other; Peter Schumann (Author) Other; Olga C Nunes (Author) FEUP; Celia M Manaia (Author) Other

Journal

Title: International Journal of Systematic and Evolutionary Microbiology

Vol. 60 No. Part 1

Pages: 2415-2419

ISSN: 1466-5026

Publisher: Microbiology Society

Indexing

ISI Web of Knowledge - 11 Citations

ISI Web of Science

Scopus - 15 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Natural sciences > Biological sciences > Biology > Microbiology

Other information

Authenticus ID: P-003-2F4

DOI: 10.1099/ijs.0.014290-0

Abstract (EN): Two bacterial strains, MC-246(T) and MC-247, were isolated from municipal urban waste compost and characterized by a polyphasic approach. Both isolates were Gram-stain-variable, endospore-forming rods that were catalase-, oxidase- and beta-galactosidase-positive, and able to grow at 25 50 degrees C and pH 7.0-9.0, with optimum growth at 37 degrees C and pH 7. The predominant cellular fatty acids were anteiso-C(15:0), iso-C(15:0), iso-C(16:0), anteiso-C(17:0) and iso-C(17:0); the major respiratory quinone was menaquinone MK-7; the cell wall peptidoglycan was of type A1 gamma; and the DNA G+C content was 49 mol%. These characteristics, as well as data from 16S RNA gene sequence analysis, showed that these strains were affiliated with the genus Paenibacillus; the type strains of Paenibacillus ginsengarvi and Paenibacillus hodogayensis were among their closest neighbours (<94.2% sequence similarity). Nevertheless, the hypothesis that strains MC-246(T) and MC-247 could represent a novel species was supported by the low 16S rRNA gene sequence similarity values shared with other members of the genus Paenibacillus and by the observation of distinct biochemical and physiological traits. Strains MC-246(T) and MC-247 shared 99.6% 16S rRNA gene sequence similarity and showed almost identical MALDI-TOF mass spectra, but could be distinguished at the phenotypic and genotypic level. However, DNA-DNA hybridization between strains MC-246(T) and MC-247 resulted in values above 70 % indicating that both organisms represent a single species, for which the name Paenibacillus residui sp. nov. is proposed; the type strain is MC-246(T) (=DSM 22072(T) =CCUG 57263(T)).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5