NO Fluorescence Sensing by Europium Tetracyclines Complexes in the Presence of H2O2

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Eliana F C Simoes (Author) Other; Joao M M Leitao (Author) Other; Joaquim C G E Esteves da Silva (Author) FCUP

Journal

Title: Journal of Fluorescence

Vol. 23

Pages: 681-688

ISSN: 1053-0509

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

ISI Web of Science

Scopus - 5 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Physical sciences > Chemistry

Other information

Authenticus ID: P-006-9ME

DOI: 10.1007/s10895-013-1207-9

Abstract (EN): The effect on the fluorescence of the europium:tetracycline (Eu:Tc), europium:oxytetracycline (Eu:OxyTc) and europium:chlortetracycline (Eu:ClTc) complexes in approximately 2:1 ratio of nitric oxide (NO), peroxynitrite (ONOO-), hydrogen peroxide (H2O2) and superoxide (O-2 (center dot-)) was assessed at three ROS/RNS concentrations levels, 30 A degrees C and pH 6.00, 7.00 and 8.00. Except for the NO, an enhancement of fluorescence intensity was observed at pH 7.00 for all the europium tetracyclines complexes-the high enhancement was observed for H2O2. The quenching of the fluorescence of the Tc complexes, without and with the presence of other ROS/RNS species, provoked by NO constituted the bases for an analytical strategy for NO detection. The quantification capability was evaluated in a NO donor and in a standard solution. Good quantification results were obtained with the Eu:Tc (3:1) and Eu:OxyTc (4:1) complexes in the presence of H2O2 200 mu M with a detection limit of about 3 mu M (Eu:OxyTc).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8