Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Pandeirada, CO (Author) Other; Maricato, E (Author) Other; Ferreira, SS (Author) Other; Correia, VG (Author) Other; Pinheiro, BA (Author) Other; Evtuguin, DV (Author) Other; Palma, AS (Author) Other; Alexandra Correia (Author) FCUP; Manuel Vilanova (Author) ICBAS; Coimbra, MA (Author) Other; Nunes, C (Author) Other

Journal

Title: Carbohydrate Polymers

Vol. 222

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 40 Citations

Scopus - 47 Citations

Other information

Authenticus ID: P-00Q-QCY

DOI: 10.1016/j.carbpol.2019.06.001

Abstract (EN): The relevance of microalgae biotechnology for producing high-value compounds with biomedical application, such as polysaccharides, has been increasing. Despite this, the knowledge about the composition and structure of microalgae polysaccharides is still scarce. In this work, water-soluble polysaccharides from Nannochloropsis oculata were extracted, fractionated, structurally analysed, and subsequently tested in terms of immunostimulatory activity. A combination of sugar and methylation analysis with interaction data of carbohydrate-binding proteins using carbohydrate microarrays disclosed the complex structural features of the different polysaccharides. These analyses showed that the water-soluble polysaccharides fractions from N. oculata were rich in (beta 1 -> 3, beta 1 -> 4)-glucans, (alpha 1 -> 3)-, (alpha 1 -> 4)-mannans, and anionic sulphated heterorhamnans. The immunostimulatory assay highlighted that these fractions could also stimulate murine B-lymphocytes. Thus, the N. oculata water-soluble polysaccharides show potential to be further explored for immune-mediated biomedical applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10