In vivo systemic toxicity assessment of an oxidized dextrin-based hydrogel and its effectiveness as a carrier and stabilizer of granular synthetic bone substitutes

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Isabel Pereira (Author) Other; Sónia Fraga (Author) Other; Luís Maltez (Author) Other; João Requicha (Author) Other; Luísa Guardão (Author) Other; Joana Oliveira (Author) Other; Justina Prada (Author) Other; Helena Alves (Author) Other; José Domingos Santos (Author) FEUP; João Paulo Teixeira (Author) Other; José Eduardo Pereira (Author) Other; Raquel Soares (Author) Other; Francisco Miguel Gama (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 107 No. 8

Pages: 1678-1689

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00Q-GS3

DOI: 10.1002/jbm.a.36683

Abstract (EN): The worldwide incidence of bone disorders is raising, mainly due to aging population. The lack of effective treatments is pushing the development of synthetic bone substitutes (SBSs). Most ceramic-based SBSs commercially available display limited handling properties. Attempting to solve these issues and achieve wider acceptance by the clinicians, granular ceramics have been associated with hydrogels (HGs) to produce injectable/moldable SBSs. Dextrin, a low-molecular-weight carbohydrate, was used to develop a fully resorbable and injectable HG. It was first oxidized with sodium periodate and then cross-linked with adipic acid dihydrazide. The in vivo biocompatibility and safety of the dextrin-based HG was assessed by subacute systemic toxicity and skin sensitization tests, using rodent models. The results showed that the HG did not induce any systemic toxic effect, skin reaction, or genotoxicity, neither impaired the bone repair/regeneration process. Then, the HG was successfully combined with granular bone substitute, registered as Bonelike (250-500 mu m) to obtain a moldable/injectable SBS, which was implanted in tibial fractures in goats for 3 and 6 weeks. The obtained results showed that HG allowed the stabilization of the granules into the defect, ensuring effective handling, and molding properties of the formulation, as well as an efficient cohesion of the granules. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1678-1689, 2019.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12