Calcium Pathways in Human Neutrophils-The Extended Effects of Thapsigargin and ML-9

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Ribeiro, D (Author) Other; Freitas, M (Author) Other; Rocha, S (Author) Other; Lima, JLFC (Author) FFUP; Felix Carvalho (Author) FFUP; Eduarda Fernandes (Author) FFUP

Journal

Title: Cells

Vol. 7

Final page: 204

ISSN: 2073-4409

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00P-Z3P

DOI: 10.3390/cells7110204

Abstract (EN): In neutrophils, intracellular Ca2+ levels are regulated by several transporters and pathways, namely SERCA [sarco(endo)plasmic reticulum Ca2+-ATPase], SOCE (store-operated calcium entry), and ROCE (receptor-operated calcium entry). However, the exact mechanisms involved in the communication among these transporters are still unclear. In the present study, thapsigargin, an irreversible inhibitor of SERCA, and ML-9, a broadly used SOCE inhibitor, were applied in human neutrophils to better understand their effects on Ca2+ pathways in these important cells of the immune system. The thapsigargin and ML-9 effects in the intracellular free Ca2+ flux were evaluated in freshly isolated human neutrophils, using a microplate reader for monitoring fluorimetric kinetic readings. The obtained results corroborate the general thapsigargin-induced intracellular pattern of Ca2+ fluctuation, but it was also observed a much more extended effect in time and a clear sustained increase of Ca2+ levels due to its influx by SOCE. Moreover, it was obvious that ML-9 enhanced the thapsigargin-induced emptying of the internal stores. Indeed, ML-9 does not have this effect by itself, which indicates that, in neutrophils, thapsigargin does not act only on the influx by SOCE, but also by other Ca2+ pathways, that, in the future, should be further explored.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13