Wheat chronic exposure to TiO2-nanoparticles: Cyto- and genotoxic approach

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Silva, S (Author) Other; Craveiro, SC (Author) Other; Oliveira, H (Author) Other; Calado, AJ (Author) Other; Pinto, RJB (Author) Other; Silva, AMS (Author) Other; Santos, C (Author) FCUP

Journal

Title: Plant Physiology and Biochemistry

Vol. 121

Pages: 89-98

ISSN: 0981-9428

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 32 Citations

Other information

Authenticus ID: P-00N-5XH

DOI: 10.1016/j.plaphy.2017.10.013

Abstract (EN): This study investigates the phytotoxicity of chronic exposure (up to 20 d) of different TiO2 nanoparticles (TiO2-NP) concentrations (5, 50, 150 mg L-1) in Triticum aestivtan, Germination was not affected by TiO2-NP exposure and seedling shoot length (3 d) was enhanced. Contrarily, plants' shoot growth (20 d) was impaired. Effects on membrane permeability and total antioxidant capacity in TiO2-NP chronic exposure were organ dependent: increased in leaves and decreased in roots. Roots also showed lower levels of lipid peroxidation. Flow cytometry revealed no changes in ploidy levels as well as in the cell cycle dynamics for both organs. However, TiO2-NP induced clastogenic effects in roots with increases in micronucleated cells in root tips in a dose dependent manner. Also, increases of DNA single/double strand breaks were found in leaves, and effects were similar to all doses. Ti uptake and translocation to leaves were confirmed by ICP-MS, which was dependent on NP concentration. Overall, these data indicate that TiO2-NP phytotoxicity is more severe after longer exposure periods, higher doses and more severe for shoots than roots. The observed effects are a result of both direct and indirect (oxidative stress and/or water imbalances) action of TiO2-NP. Additionally, results highlight the negative impact that TiO2-NP may have on crop growth and production and to the risk of trophic transfer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10