Different Escherichia coli B2-ST131 clades (B and C) producing extended-spectrum beta-lactamases (ESBL) colonizing residents of Portuguese nursing homes

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Rodrigues, C (Author) Other; Machado, E (Author) Other; Fernandes, S (Author) Other; Luisa Peixe (Author) FFUP; Novais, A (Author) Other

Journal

Title: Epidemiology and Infection

Vol. 145

Pages: 3303-3306

ISSN: 0950-2688

Publisher: Cambridge University Press

Indexing

ISI Web of Knowledge - 0 Citations

Other information

Authenticus ID: P-00N-7YM

DOI: 10.1017/s0950268817002266

Abstract (EN): ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9.5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods. Clonal structure and plasmid typing of ESBL-producing E. coli (ESBL-Ec) was performed by PCR and sequencing. Four ESBL-Ec isolates (2 CTX-M-15/2 CTX-M-14) were found in 20% of the samples, all belonging to the pandemic clonal lineage B2-ST131-O25b:H4. Two different clades were identified, the C2/H30-Rx-virotype C producing CTX-M-15 and an atypical B/H22-like-virotype D5 (producing CTX-M-14 and fluoroquinolone-resistant), firstly described in Portugal. This pilot study highlights the role of NH residents as a source of different ST131 clades, besides emphasizing the importance of E. coli B2-ST131 subtyping in different clinical settings, and understanding the transmission dynamics of the different variants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4