Surface characterization and cell response of a PLA/CaP glass biodegradable composite material

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Navarro, M (Author) Other; Engel, E (Author) Other; Planell, JA (Author) Other; Amaral, IF (Author) Other; Mário Adolfo Barbosa (Author) FEUP; Ginebra, MP (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 85A

Pages: 477-486

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 13 Citations

Other information

Authenticus ID: P-003-ZR0

DOI: 10.1002/jbm.a.31546

Abstract (EN): Bioabsorbable materials are of great interest for bone regeneration applications, since they are able to degrade gradually as new tissue is formed. In this work, a fully biodegradable composite material containing polylactic acid (PLA) and calcium phosphate (CaP) soluble glass particles has been characterized in terms of surface properties and cell response. Cell cultures were performed in direct contact with the materials and also with their extracts, and were evaluated using the MTT assay, alkaline phosphatase activity, and osteocalcin measurements. The CaP glass and PLA were used as reference materials. No significant differences were observed in cell proliferation with the extracts containing the degradation by-products of the three materials studied. A relation between the materials wettability and the material-cell interactions at the initial stages of contact was observed. The most hydrophilic material (CaP glass) presented the highest cell adhesion values as well as an earlier differentiation, followed by the PLA/glass material. The incorporation of glass particles into the PLA matrix increased surface roughness. SEM images showed that the heterogeneity of the composite material induced morphological changes in the cells cytoskeleton. (C) 2007 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10