Development of high performance and facile to pack molecularly imprinted particles for aqueous applications

• Type: Article in International Scientific Journal
• Date: 2016

Authors

M. R. P. F. N. Costa (Author) FEUP; R. C. S. Dias (Author) Other; D. Oliveira (Author) Other; P. Kadhirvel (Author) Other; A. Freitas (Author) Other

Journal

Title: Biochemical Engineering Journal

Vol. 111

Pages: 87-99

ISSN: 1369-703X

Publisher: Elsevier

Associated Projects

LSRE-LCM - Laboratório de Processos de Separação e Reação - Laboratório de Catálise e Materiais

Other information

DOI: 10.1016/j.bej.2016.03.009

Abstract (EN): Different kinds of molecularly imprinted particles were synthesized and compared, aiming at the development of materials combining high molecular recognition capabilities and facile use as column packing materials for chromatographic aqueous applications. Solution, inverse-suspension and precipitation polymerization were considered and two different model molecules (5-fluorouracil and caffeine) were used to highlightthe effect ofthe interaction between the template molecule and the functional monomer on imprinting efficiency. Particles synthesized through the proposed inverse-suspension process exhibit facile use for packing columns, allow the stable running of chromatographic systems and present a high performance in drug uptake and release in aqueous media. Frontal analysis measurements highlightthese key features of the synthesized particles. Drug sorption capabilities of 0.890 mol/g and 5.774 mol/g were measured for 5-fluorouracil and caffeine, respectively, using frontal analysis with eluents containing the target molecules at concentration 0.1 mM. Due to the lower amount of solvent required than with precipitation polymerization, the developed inverse-suspension process presents high synthesis yields, which can be exploited for the large-scale manufacture and commercialization of molecularly imprinted materials. The combined features of the particles makes possible their direct use in bioseparations or in the development of assays and pharmacokinetic studies concerning the presence of drugs in biological fluids.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13