Properties and partial characterization of the heat-shock factor from Tetrahymena pyriformis

• Type: Article in International Scientific Journal
• Date: 1990

Authors

Do Carmo Avides, M (Author) Other; Sunkel, CE (Author) Other; Pedro Moradas Ferreira (Author) ICBAS; Rodrigues Pousada, C (Author) Other

Journal

Title: European Journal of Biochemistry

Vol. 194

Pages: 331-336

ISSN: 0014-2956

Publisher: Blackwell

Indexing

Scopus - 11 Citations

Other information

Authenticus ID: P-00F-GN8

Abstract (EN): A heat-shock-factor-binding activity was identified in Tetrahymena pyriformis whole-cell extracts and was further purified by sequential heparin-agarose and sequence-specific oligonucleotide affinity chromatography. Tetrahymena heat-shock factor (HSF) was able to bind to the heat-shock elements (HSE) both before and after thermal stress, although heat shock altered both the HSE-binding affinity and the protein · DNA-complex mobility on polyacrylamide gels. The mobility difference was significantly reduced by treatment of the proteins with phosphatase. The HSE-binding proteins, isolated by oligonucleotide-affinity chromatography, migrated on SDS/polyacrylamide gels as a closely spaced doublet to about 70 kDa. Polypeptides with similar molecular mass were recovered from preparative band-shift gels indicating that both are components of the protein · DNA complex.

Language: English

Type (Professor's evaluation): Scientific