Survey of Klebsiella pneumoniae producing extended-spectrum beta-lactamases at a Portuguese hospital: TEM-10 as the endemic enzyme

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Barroso, H (Author) Other; Freitas Vieira, A (Author) Other; Lito, LM (Author) Other; Cristino, JM (Author) Other; Salgado, MJ (Author) Other; Neto, HF (Author) FFUP; Sousa, JC (Author) Other; Soveral, G (Author) Other; Moura, T (Author) Other; Duarte, A (Author) Other

Journal

Title: Journal of Antimicrobial Chemotherapy

Vol. 45

Pages: 611-616

ISSN: 0305-7453

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 31 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-001-060

DOI: 10.1093/jac/45.5.611

Abstract (EN): One hundred and thirty-eight isolates of Klebsiella pneumoniae showing resistance to ceftazidime were isolated from different wards of the Hospital de Santa Maria, Lisbon, The genomic DNA of the isolates was analysed by pulsed-field gel electrophoresis (PFGE) and two patterns were predominant. In all isolates the presence of a single large plasmid of about 50 kb suggested that propagation of the outbreak prominently involved plasmid spread, The deduced amino acid sequence indicated the presence of a TEM-10 beta-lactamase. This extended-spectrum P-lactamase was present among K. pneumoniae isolates, was widely disseminated in different wards and remained persistent as a result of an outbreak involving the dissemination of both the multi-resistance plasmids harbouring the bla gene and the isolates themselves.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6