Monosegmented flow-analysis of serum cholesterol

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Araujo, AN (Author) FFUP; Catita, JAM (Author) Other; Lima, JLFC (Author) FFUP

Journal

Title: Farmaco

Vol. 54

Pages: 51-55

ISSN: 0014-827X

Publisher: Elsevier B.V.

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 9 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-001-5MH

DOI: 10.1016/s0014-827x(98)00093-7

Abstract (EN): A monosegmented flow system is designed for enzymatic spectrophotometric determination of cholesterol in blood serum. The sample (4.5 mu l), enzymatic reagent (150 mu l) and an air plug (100 mu l) are simultaneously inserted into a carrier stream buffered to pH 7.4 (potassium dihydrogenphosphate). In order to avoid the step of air removal, a relocating detector was used. This system handles about 42 samples per hour, yielding precise results (R.S.D. usually < 3.0%). Sensitivity is 46 mAU l/mmol (mAU stands for milliabsorbance units), being the method linear up to 10.3 mmol/l cholesterol. Accuracy was assessed by running 30 samples already analysed by a conventional procedure: no statistical difference between methods was found at the 95% confidence level.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5