Survey of 548 oncogenic fusion transcripts in thyroid tumors supports the importance of the already established thyroid fusions genes

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Ricardo Celestino (Author) Other; Eva Sigstad (Author) Other; Marthe Lovf (Author) Other; Gard O S Thomassen (Author) Other; Krystyna K Groholt (Author) Other; Lars H Jorgensen (Author) Other; Aasmund Berner (Author) Other; Patricia Castro (Author) FMUP; Ragnhild A Lothe (Author) Other; Trine Bjoro (Author) Other; Manuel Sobrinho Simoes (Author) FMUP; Paula Soares (Author) FMUP; Rolf I Skotheim (Author) Other

Journal

Title: Genes Chromosomes and Cancer

Vol. 51

Pages: 1154-1164

ISSN: 1045-2257

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 14 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

Other information

Authenticus ID: P-002-37E

DOI: 10.1002/gcc.22003

Abstract (EN): Neoplasms frequently present structural chromosomal aberrations that can alter the level of expression of a protein or to the expression of an aberrant chimeric protein. In the thyroid, the PAX8-PPARG fusion is present in the neoplastic lesions that have a follicular architecturefollicular thyroid carcinoma (FTC) and follicular variant of papillary thyroid carcinoma (FVPTC), and less frequently in follicular thyroid adenoma (FTA), while the presence of RET/PTC fusions are largely restricted to papillary thyroid carcinoma (PTC). The ability to detect fusion genes is relevant for a correct diagnosis and for therapy. We have developed a new fusion gene microarray-based approach for simultaneous analysis of all known and predicted fusion gene variants. We did a comprehensive screen for 548 known and putative fusion genes in 27 samples of thyroid tumors and three positive controlsone thyroid cancer cell line (TPC-1) and two PTCs with known CCDC6-RET (alias RET/PTC1) fusion gene, using this microarray. Within the thyroid tumors tested, only well known, previously reported fusion genes in thyroid oncology were identified. Our results reinforce the pathogenic role played by RET/PTC1, RET/PTC3, and PAX8-PPARG fusion genes in thyroid tumorigenesis. (c) 2012 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11