Studies on the hemocompatibility of bacterial cellulose

• Type: Article in International Scientific Journal
• Date: 2011

Authors

andrade, fk (Author) Other; silva, jp (Author) Other; carvalho, m (Author) Other; castanheira, ems (Author) Other; soares, r (Author) FMUP; gama, m (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 98A

Pages: 554-566

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 89 Citations

Scopus - 90 Citations

Other information

Authenticus ID: P-002-NEY

DOI: 10.1002/jbm.a.33148

Abstract (EN): Among the strategies to improve a material's hemocompatibility, pre-coating with the tripeptide Arg-Gly-Asp (RGD) is used to favor endothelialization thus lowering thrombogenicity. The blood compatibility of native and RGD-modified bacterial cellulose (BC) was studied in this work for the first time. The plasma recalcification time and whole blood clotting results demonstrate the hemocompatibility of BC. A significant amount of plasma protein adsorb to BC fibres, however, according to analysis by intrinsic tryptophan fluorescence techniques when albumin, gamma-globulin, and fibrinogen from pure protein solutions adsorb to BC do not undergo detectable conformational modifications. Human microvascular endothelial cells cultured on RGD-modified BC readily form a confluent cell layer, inhibiting the adhesion of platelets. As a general conclusion, both native and RGD-modified BCs may be classified as hemocompatible materials. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 98A: 554-566, 2011.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13