Non-transferrin-bound iron determination in blood serum using microsequential injection solid phase spectrometry- proof of concept

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Miranda, JLA (Author) Other; Mesquita, RBR (Author) Other; Leite, A (Author) Other; Silva, AMN (Author) Other; Maria Rangel (Author) ICBAS; Rangel, AOSS (Author) Other

Journal

Title: Talanta

Vol. 257

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus

Other information

Authenticus ID: P-00X-WA0

DOI: 10.1016/j.talanta.2023.124345

Abstract (EN): Non-transferrin-bound iron (NTBI) is a group of circulating toxic iron forms, which occur in iron overload or health conditions with dysregulation of iron metabolism. NTBI is responsible for increased oxidative stress and tissue iron loading. Despite its relevance as a biochemical marker in several diseases, a standardized assay is still lacking. Several methods were developed to quantify NTBI, but results show high inter-method and even inter -laboratory variability. Thus, the development of a consistent NTBI assay is a major goal in the management of iron overload and related clinical conditions. In this work, a micro sequential injection lab-on-valve (mu SI-LOV) method in a solid phase spectrophotometry (SPS) mode was developed for the quantification of NTBI, using a bidentate 3,4-hydroxypyridinone (3,4-HPO) ligand anchored to sepharose beads as a chromogenic reagent. To attain SPS, the functionalized beads were packed into a column in the flow cell, and the analyte, NTBI retained as iron (III), formed a colored complex at the beads while eliminating the sample matrix. The dynamic concen-tration range was 1.62-7.16 mu mol L-1 of iron (III), with a limit of detection of 0.49 mu mol L-1 and a limit of quantification of 1.62 mu mol L-1. The proposed mu SI-LOV-SPS method is a contribution to the development of an automatic method for the quantification of the NTBI in serum samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7