Alienimonas chondri sp. nov., a novel planctomycete isolated from the biofilm of the red alga Chondrus crispus

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Vitorino, I (Author) Other; Albuquerque, L (Author) Other; Wiegand, S (Author) Other; Kallscheuer, N (Author) Other; da Costa, MS (Author) Other; Lobo da Cunha, A (Author) Other; Jogler, C (Author) Other; lage, om (Author) FCUP

Journal

Title: Systematic and Applied Microbiology

Vol. 43

ISSN: 0723-2020

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-00S-20Z

DOI: 10.1016/j.syapm.2020.126083

Abstract (EN): The phylum Planctomycetes comprises bacteria with peculiar and very unique characteristics among prokaryotes. In marine environments, macroalgae biofilms are well known for harboring planctomycetal diversity. Here, we describe a novel isolate obtained from the biofilm of the red alga Chondrus crispus collected at a rocky beach in Porto, Portugal. The novel strain LzC2(T) is motile, rosette-forming with spherical- to ovoid-shaped cells. LzC2(T) forms magenta- to pinkish-colored colonies in M13 and M14 media. Transmission and scanning electron microscopy observations showed a division by polar and lateral budding. Mother cells are connected to the daughter cells by a tubular neck-like structure. The strain requires salt for growth. Vitamins are not required for growth. Optimal growth occurs from 15 to 30 degrees C and within a pH range from 5.5 to 10.0. Major fatty acids are anteiso-C15:0 (54.2%) and iso-C15:0 (19.5%). Phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid represent the main lipids and menaquinone 6 (MK-6) is the only quinone present. 16S rRNA gene-based phylogenetic analysis supports the affiliation to the phylum Planctomycetes and family Planctomycetaceae, with Alienimonas as the closest relative. Strain LzC2(T) shares 97% 16S rRNA gene sequence similarity with Alienimonas californiensis. LzC2(T) has a genome size of 5.3 Mb and a G+C content of 68.3%. Genotypic and phenotypic comparison with the closest relatives strongly suggest that LzC2(T) (=CECT 30038(T)=LMG XXXT) is a new species of the genusAlienimonas, for which we propose the nameAlienimonas chondri sp. nov., represented by LzC2(T) as type strain.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8