Starch-Capped AgNPs' as Potential Cytotoxic Agents against Prostate Cancer Cells

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Morais, M (Author) Other; Machado, V (Author) Other; Dias, F (Author) Other; Palmeira, C (Author) Other; Martins, G (Author) Other; Fonseca, M (Author) Other; Martins, CSM (Author) Other; Teixeira, AL (Author) Other; Joao A V Prior (Author) FFUP; Rui Medeiros (Author) ICBAS

Journal

Title: Nanomaterials

Vol. 11

Pages: 1-17

ISSN: 2079-4991

Publisher: MDPI

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00T-BQP

DOI: 10.3390/nano11020256

Abstract (EN): One of the major therapeutic approaches of prostate cancer (PC) is androgen deprivation therapy (ADT), but patients develop resistance within 2-3 years, making the development of new therapeutic approaches of great importance. Silver nanoparticles (AgNPs) synthesized through green approaches have been studied as anticancer agents because of their physical-chemical properties. This study explored the cytotoxic capacity of starch-capped AgNPs, synthesized through green methods, in LNCaP and in PC-3 cells, a hormonal-sensitive and hormone-resistant PC cell line, respectively. These AgNPs were synthesized in a microwave pressurized synthesizer and characterized by ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Their cytotoxicity was assessed regarding their ability to alter morphological aspect (optical microscopy), induce damage in cytoplasmic membrane (Trypan Blue Assay), mitochondria (WST-1 assay), cellular proliferation (BrdU assay), and cell cycle (Propidium iodide and flow-cytometry). AgNPs showed surface plasmon resonance (SPR) of approximately 408 nm and average size of 3 nm. The starch-capped AgNPs successfully induced damage in cytoplasmic membrane and mitochondria, at concentrations equal and above 20 ppm. These damages lead to cell cycle arrest in G0/G1 and G2/M, blockage of proliferation and death in LNCaP and PC-3 cells, respectively. This data shows these AgNPs' potential as anticancer agents for the different stages of PC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17