POLAROGRAPHIC DETERMINATION OF VITAMIN C AFTER DERIVATIZATION WITH o-PHENYLENEDIAMINE

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Jose Antonio Rodrigues (Author) FCUP; Ines Maria Valente (Author) Other; Luis Moreira Goncalves (Author) Other; Joao Grosso Pacheco (Author) Other; Aquiles Araujo Barros (Author) FCUP

Journal

Title: Collection of Czechoslovak Chemical Communications

Vol. 75 No. 7

Pages: 731-741

ISSN: 0010-0765

Publisher: Akademie Ved Ceske Republiky

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Physical sciences > Chemistry > Instrumental analysis ; Physical sciences > Chemistry > Applied chemistry

Other information

Authenticus ID: P-003-AEK

DOI: 10.1135/cccc2010026

Abstract (EN): A differential pulse polarographic (DPP) method has been developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHA), the two main forms of Vitamin C. The method consists of the DPP analysis of a quinoxaline obtained by the derivatization of DHA with o-phenylenediamine. Results using the proposed method correlated well with those obtained by two reference methodologies: the common iodometric method and a published chromatographic methodology. It was also used in the study of Vitamin C degradation in fruit juices, showing that it involves an initial oxidation of AA to DHA, followed by hydrolytic degradation of the latter.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11