Screen-printed Electrode Based Electrochemical Sensor for the Detection of Isoniazid in Pharmaceutical Formulations and Biological Fluids

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Couto, RAS (Author) Other; Lima, JLFC (Author) FFUP; M. Beatriz Q. Garcia (Author) FFUP

Journal

Title: International Journal of Electrochemical Science

Vol. 10

Pages: 8738-8749

ISSN: 1452-3981

Publisher: Electrochemical Science Group, University of Belgrade

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00G-TP4

Abstract (EN): This work focuses on the development of a novel and sensitive electrochemical sensor for the tuberculostatic drug INH. The use of INH is frequently associated to numerous side effects and poisoning incidents, being the assay of INH levels essential for effective therapeutic dosages. Herein we propose an electrochemical method based on the use of Nafion modified-SPCEs for the study of the electro-oxidation process of INH and its determination in pharmaceutical formulations and biological fluids. The modified electrodes showed high stability, good sensitivity and reproducibility, enabling the use of micro-volumes of samples for the determination of the drug in complex matrixes. The study of the electrochemical behaviour of INH was performed by CV and SWV and the method was successfully applied to the determination of the drug in tablets and in human urine and serum samples in 0.1M PBS pH 7.0 using 100 mu L of samples and no pre-treatment steps. The proposed method exhibited numerous advantages, such as simplicity, rapid analysis procedures and great sensitivity, offering a promising alternative for the analysis of INH using disposable Nafion-SPCE that can be used in portable sensors for decentralized analyses of this analyte.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12