Metabolic Control Analysis and its Applications

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ana Meireles (Author) FEUP; Manuel Simões (Author) FEUP

Journal

Title: Current Bioinformatics

Vol. 9 No. 5

Pages: 490-498

ISSN: 1574-8936

Publisher: Bentham

Indexing

ISI Web of Science

Scopus

Pubmed / Medline

Scientific classification

CORDIS: Technological sciences

FOS: Engineering and technology

Associated Projects

UI0511 - PROJECTO ESTRATÉGICO - UI 511 - 2013-2014

Other information

DOI: 10.2174/1574893609666140515230435

Abstract (EN): Metabolic Engineering (ME) provides the know-how for the reconstruction of 3 microorganisms, in order to provide higher production rates of biotechnological 4 products and increase their potential application in several industries, particularly those 5 pharmaceutical, food and environmental. However, microbial metabolic cell 6 reconstruction has limitations. Metabolic Control Analysis (MCA) allows the evaluation 7 of the reliability of the changes performed by ME, emphasizing the importance of the 8 whole pathways rather than individual pathway reactions resulting from simple flux 9 analysis. This led to an increased emphasis on the regulatory structure of the network. 10 The use of MCA becomes indispensable to quantify metabolic parameters, particularly 11 those related with direct genetic modifications. However, this type of analysis is not a 12 common practice. In the present study MCA is exemplified as a tool of ME, being 13 demonstrated its practical application in drug delivery and in the production of three 14 relevant biotechnological products (penicillin V, l-lysine and glycerol).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9