Methodological aspects about in vitro evaluation of antioxidant properties

• Type: Another Publication in an International Scientific Journal
• Date: 2008

Authors

Luis M Magalhaes (Author) Other; Marcela A Segundo (Author) FFUP; Salette Reis (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Analytica Chimica Acta

Vol. 613 No. 1

Pages: 1-19

ISSN: 0003-2670

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 415 Citations

Scopus - 531 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-060

DOI: 10.1016/j.aca.2008.02.047

Abstract (EN): Several of the most commonly used methods for in vitro determination of antioxidant capacity are reviewed in the present paper. The chemical principles of methods based either on biological oxidants (peroxyl radical, superoxide radical anion, hydrogen peroxide, hydroxyl radical, hypochlorous acid, singlet oxygen, nitric oxide radical, and peroxynitrite) or on non-biological assays (scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (TEAC assay), scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH degrees assay), ferric reducing antioxidant power (FRAP assay), Folin-Ciocalteu reducing capacity (FC assay), electrochemical total reducing capacity) are outlined and critically discussed. The scope of application, the advantages and shortcomings of each method are also highlighted.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19