Primary structure, in vitro expression and import into mitochondria of a 29 21-KDA subunit of complex I from Neurospora crassa

• Tipo: Artigo em Revista Científica Internacional
• Data: 1990

Autores

videira, a (Autor) ICBAS; tropschug, m (Autor) Outra; werner, s (Autor) Outra

Revista

Título: Biochemical and Biophysical Research Communications

Vol. 166

Páginas: 280-285

ISSN: 0006-291X

Editora: Elsevier

Indexação

ISI Web of Knowledge

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-008-NM0

Abstract (EN): A full-length cDNA clone coding for a cytoplasmically-synthesized subunit of complex I from Neurospora crassa (apparent molecular mass of 29 kDa) was isolated. DNA sequencing revealed an open reading frame coding for a protein containing 201 amino acids. A molecular mass of 21323 Da was calculated. The precursor polypeptide was efficiently expressed in vitro and imported into isolated mitochondria. It is synthesized without a cleavable signal sequence and needs a membrane potential in order to bind to the mitochondrial membranes. © 1990.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6