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Primary structure, in vitro expression and import into mitochondria of a 29 21-KDA subunit of complex I from Neurospora crassa

Title
Primary structure, in vitro expression and import into mitochondria of a 29 21-KDA subunit of complex I from Neurospora crassa
Type
Article in International Scientific Journal
Year
1990
Authors
videira, a
(Author)
ICBAS
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tropschug, m
(Author)
Other
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werner, s
(Author)
Other
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Journal
Vol. 166
Pages: 280-285
ISSN: 0006-291X
Publisher: Elsevier
Indexing
Publicação em ISI Web of Knowledge ISI Web of Knowledge
Other information
Authenticus ID: P-008-NM0
Abstract (EN): A full-length cDNA clone coding for a cytoplasmically-synthesized subunit of complex I from Neurospora crassa (apparent molecular mass of 29 kDa) was isolated. DNA sequencing revealed an open reading frame coding for a protein containing 201 amino acids. A molecular mass of 21323 Da was calculated. The precursor polypeptide was efficiently expressed in vitro and imported into isolated mitochondria. It is synthesized without a cleavable signal sequence and needs a membrane potential in order to bind to the mitochondrial membranes. © 1990.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 6
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