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Project/Service Agreement:POCI-01-0145-FEDER-029589

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Status
Estado ConcluídoCompleted
Publication
PublicadoPublished
General Data
Code: 73319
 
Reference: POCI-01-0145-FEDER-029589
Short name: FilmProt
Title: The use of Escherichia coli biofilms for the production of recombinant proteins and other added-value compounds
Competitive Funding: Yes
Does it involve businesses?: No
No. of Participating Institutions: 2
Scope
Type: Funded Project
 
Geographical Scope: National
 
Type of Action: R&TD
Funding
Programme: P2020|COMPETE - Projetos em Todos os Domínios Científicos
Funding Institution: FCT - Fundação para a Ciência e a Tecnologia
Financial Geographical Scope: National
Date of the Funding Agreement: 2018-07-27
Paying Entity: Fundação para a Ciência e a Tecnologia
Scheduling
Effective Start Date: 2018-10-01
Expected Completion Date: 2021-09-30
Effective Completion Date: 2022-05-31
Budget
Currency: EUR
 
Total Approved Budget: 239.820,97 EUR
Details
Summary: Research in microbial biofilms is usually focused on their deleterious effects in the health and industrial sectors.
Nevertheless, microbial biofilms are used advantageously in wastewater treatment systems or for the production of
valuable compounds since biofilm reactors are able to retain more biomass than suspended cell reactors.
E. coli biofilms have a great potential since this organism has been one of the preferred hosts for recombinant protein
production for the past decades and it has been successfully used in metabolic engineering for the production of
high value compounds. Despite this success, recombinant protein production using E. coli biofilms has been scarcely
investigated for the past 25 years. The FEUP group has recently demonstrated that E. coli biofilms can be considered
as an alternative form of high cell density cultivation and that even without the optimization of culture conditions it was
possible to attain an attractive production level of the model green fluorescent protein (GFP).
This project has two main objectives, the first is to optimize the culture conditions in order to determine the maximum
specific and volumetric productions that can be attained with this biofilm system and the second is to investigate the
robustness of the system. The significance of this project is not only to establish E. coli biofilm systems has a versatile
platform for recombinant protein production but also to better understand what is the performance of these systems
in the expression of recombinant enzymes that can be used for metabolic engineering of single cells or even in cell
coculture for more complex biosynthetic pathways.
Results: Os principais outputs do projeto foram: Papers in international journals • Soares A, Azevedo A, Gomes LC, Mergulhão FJ. Recombinant protein expression in biofilms. AIMS Microbiol. 2019 27;5(3):232-250. doi: 10.3934/microbiol.2019.3.232. • Gomes L, Monteiro G, Mergulhão F. The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms. Int J Mol Sci. 2020 16;21(2):576. doi: 10.3390/ijms21020576. • Soares A, Gomes LC, Monteiro GA, Mergulhão FJ. The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression. Appl. Sci. 2021; 11(18):8667. doi: 10.3390/app11188667. • Gomes LC, Mergulhão FJM. A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions. Microorganisms 2021; 9(9):1993. doi: 10.3390/microorganisms9091993. • Gomes LC, Ferreira C, Mergulhão FJ. Implementation of a Practical Teaching Course on Protein Engineering. Biology 2022; 11(3):387. https://doi.org/10.3390/biology11030387 • Soares A, Gomes LC, Monteiro GA, Mergulhão FJ. Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression. Microorganisms 2022; 10(5):931. https://doi.org/10.3390/microorganisms10050931 • Carvalho FM, Azevedo A, Ferreira MM, Gomes LC, Mergulhão FJ. Advances on Bacterial and Fungal Biofilms for the production of value-added compounds. (In preparation) • Azevedo A, Carvalho FM, Gomes LC, Monteiro GA, Mergulhão FJ. Impact of surface material and nutrient conditions on Green Fluorescent Protein (GFP) production in Escherichia coli biofilms. Biology. (Submitted) • Soares, A, Carvalho, FM, Gomes, LC, Monteiro GA, Mergulhão FJ. Effect of IPTG induction on specific GFP production by E. coli biofilms. (In preparation) • Azevedo, A., Carvalho FM, Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Production of curcumin in biofilms developed under controlled flow conditions. (In preparation) Book chapters • Luciana Gomes and Filipe José Mergulhão. "Production of Recombinant Proteins in Escherichia coli Biofilms: Challenges and Opportunities". In Advances in Medicine and Biology, 183-217. Nova Science Publishers, Inc., 2019. • Azevedo, A., F.M. Carvalho, L.C. Gomes, and F.J. Mergulhão. “Use of Bacterial Biofilms to Produce High Added-Value Compounds”, in Understanding microbial biofilms: Fundamentals to applications, S. Das and N. Kungwani, Editors. 2022, Academic Press. ISBN: 9780323999779. Communications in international meetings • Gomes, L.C., Alves, P., Soares, A., Mergulhão, F.J. Microscopy investigation of bacterial biofilm development in flow systems. INTERM 2019 — 6th International Symposium on Microscopy and Spectroscopy, May 2019, Turkey (oral presentation). • Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Recombinant Protein Production and Plasmid Stability in Escherichia coli Biofilms. Biofilms 9, 2020. doi: 10.5194/biofilms9-65 (oral presentation, online). • Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Surface and nutrient media: impact on E. coli biofilms expressing Green Fluorescent Protein (GFP). Biointerfaces International Zurich 2021, August 2021, (poster presentation, online). http://www.biointerfaces.ch/international/2021/ • Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Heterologous protein expression in E. coli biofilms: gene dosage and induction effects. Biointerfaces International Zurich 2021, August 2021 (poster presentation, online).
Observations: continuação dos outputs (apresentações orais em conferências internacionais) • Gomes, L.C., Monteiro, G.A., Mergulhão, F.J. Biofilm systems as tools in the production of recombinant proteins and other added-value compounds. 3rd Aachen Protein Engineering Symposium, September 2021 (flash presentation, online). • Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Expression of Green Fluorescent Protein (GFP) in Escherichia coli biofilms: impact of surface and nutrient conditions. International Biodeterioration & Biodegradation Symposium (IBBS18), September 2021 (poster presentation, online). https://www.ibbs18.org/ • Soares, A., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Effect of nutrient medium composition on heterologous protein production in biofilms. International Biodeterioration & Biodegradation Symposium (IBBS18), September 2021 (poster presentation, online). https://www.ibbs18.org/ • Soares, A., Gomes, L.C., Mergulhão F.J. Using microscopy to assess the effect of medium composition during recombinant protein production in biofilms. INTERM 2022 — 9th International Symposium on Microscopy and Spectroscopy, April 2022, Turkey (oral presentation). • Carvalho, F.M., Azevedo, A., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. Expression of recombinant Green Fluorescent Protein (GFP) using Escherichia coli biofilms: effects of culture media and surface properties. Biofilms 10, May 2022 (poster presentation, online). https://www.ufz.de/index.php?en=49335 • Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A, Mergulhão F.J. Green Fluorescent Protein (GFP) expression in Escherichia coli biofilms: effects of surface and nutrient conditions. SurfSAFE Scientific Workshop 1: Characterization of non-biotoxic antimicrobial, anti-adhesive and biomimetic surfaces, June 2021 (oral presentation, online). • Azevedo, A., Carvalho, F.M., Gomes, L.C., Monteiro, G.A, Mergulhão F.J. The influence of surface and media composition on the expression of Green Fluorescent Protein (GFP) in Escherichia coli biofilms. SurfSAFE Scientific Workshop 2: Protein adsorption and biofilm formation, December 2021, Netherlands (oral presentation). Communications in national meetings • Soares, A., Gomes, L.C., Mergulhão F.J. Effect of nutrient medium composition on the expression of a recombinant protein in Escherichia coli biofilms". Doctoral Congress in Engineering 2019 - DCE 2019, 2019, FEUP, Portugal (poster presentation). https://paginas.fe.up.pt/~dce/2019/programme/poster-session/. • Azevedo, A., Gomes, L.C., Teixeira-Santos, R., Monteiro, G.A., Mergulhão F.J., Optimization of enhanced Green Fluorescent Protein (eGFP) production in Escherichia coli biofilms. DCE 21 - 4th Doctoral Congress in Engineering, June 2021 (poster presentation, online). https://paginas.fe.up.pt/~dce/2021/. Organization of seminars and conferences • Scientific Workshop 1 – “Characterization of non-biotoxic, antimicrobial, anti-adhesive and biomimetic surfaces”, June 2021 (online). • Scientific Workshop 2 – “Protein adsorption and biofilm formation”, December 2021, Groningen, Netherlands. • Training School 1 – “Evaluation of the surface performance”, March 2022, Porto, Portugal. PhD thesis • Alexandra Soares, “Optimizing Recombinant Protein Production in Escherichia coli Biofilms” (FCT, Ref. SFRH/141614/2018) (end in September 2022) • Ana Azevedo, “The use of Escherichia coli biofilms for the production of recombinant proteins and other added-value compounds”
Scientific Context
Scientific Domain (FOS - Level 2): Engineering and technology

Academic fields (CORDIS - Level 5)

Mais informações There are no Academic Fields associated with the Project.

Keywords

Mais informações There are no Keywords associated with the Project.
Documents
Mais informações There are no Documents associated with the Project.

Publications associated with the Project

Implementation of a Practical Teaching Course on Protein Engineering
Article in International Scientific Journal
Luciana C. Gomes (Author) (FEUP); Carla Ferreira (Author) (Other); Filipe Mergulhão (Author) (FEUP)
2022
Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression
Article in International Scientific Journal
Soares, A (Author) (Other); Luciana Gomes (Author) (FEUP); Monteiro, GA (Author) (Other); Filipe Mergulhão (Author) (FEUP)
2022
Carbon Nanotube-Based Antimicrobial and Antifouling Surfaces
Chapter or Part of a Book
Rita Teixeira-Santos (Author) (FEUP); Marisa Gomes (Author) (FEUP); Filipe Mergulhão (Author) (FEUP)
2020
Use of bacterial biofilms to produce high added-value compounds
Chapter or Part of a Book
Ana Azevedo (Author) (FEUP); Fábio M. Carvalho (Author) (FEUP); Luciana Gomes (Author) (FEUP); Filipe Mergulhão (Author) (FEUP)
2022
Advances on Bacterial and Fungal Biofilms for the Production of Added-Value Compounds
Another Publication in an International Scientific Journal
Carvalho, FM (Author) (Other); Azevedo, A (Author) (Other); Ferreira, MM (Author) (Other); Filipe Mergulhão (Author) (FEUP); Luciana Gomes (Author) (FEUP)
2022
Curcumin production by engineered Escherichia coli biofilms
Poster in an International Conference
Luciana Gomes (Author) (FEUP); Ana Azevedo (Author) (Other); Marta Ferreira (Author) (Other); Gabriel Monteiro (Author) (Other); Filipe Mergulhão (Author) (FEUP)
2023
Institutions Participating in the Project
Institution Contact Create Tab?
Name Short name Country Type Participation Name Telephone Email
Faculdade de Engenharia da Universidade do Porto FEUP Portugal University Proponent
Associação do Instituto Superior Técnico para a Investigação e o Desenvolvimento IST-ID Portugal Non-profit institution Partner
 
Budgets and Teams
Approved Budget: 204.820,97 EUR
Approved Funded Amount: 204.820,97 EUR
Approved co-funded Amount: 0,00 EUR
Funding Rate: 100 %
Confidential Budget:

People in the Project

Institution Name Short name Role Dedication (%) Contribution (%) Allocation
Start date End date
FEUP Ana Cláudia Moreira Azevedo ACMA Grant recipient 100 2019-03-12 2020-12-31
FEUP Fábio Rafael Moreira Carvalho FRMC Grant recipient 100 2021-02-15 2022-05-31
FEUP Filipe José Menezes Mergulhão FJMM Official Researcher at the OU 2018-10-01 2022-03-31
FEUP Rita Daniela Teixeira dos Santos RDTS Researcher 100 2019-07-29 2022-05-31

Technicians in the Project

Technician Contact
FEUP 624190 Maria de Lurdes Fernandes de Matos Gonçalves
Laboratories
Mais informações There are no Laboratories associated with the Project.
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