Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Joana S AMARAL (Autor) Outra; Susana Casal (Autor) FFUP; Duarte TORRES (Autor) Outra; Rosa M SEABRA (Autor) Outra; Beatriz P P OLIVEIRA (Autor) FFUP

Revista

Título: Analytical Sciences

Vol. 21 Nº 4

Páginas: 1545-1548

ISSN: 0910-6340

Editora: Japan Society for Analytical Chemistry

Indexação

ISI Web of Knowledge - 83 Citações

Scopus - 97 Citações

Outras Informações

ID Authenticus: P-000-03W

DOI: 10.2116/analsci.21.1545

Abstract (EN): A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E homologues.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4