Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Joana S AMARAL (Author) Other; Susana Casal (Author) FFUP; Duarte TORRES (Author) Other; Rosa M SEABRA (Author) Other; Beatriz P P OLIVEIRA (Author) FFUP

Journal

Title: Analytical Sciences

Vol. 21 No. 4

Pages: 1545-1548

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 83 Citations

Scopus - 97 Citations

Other information

Authenticus ID: P-000-03W

DOI: 10.2116/analsci.21.1545

Abstract (EN): A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E homologues.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4