Rational development of molecular imprinted carbon paste electrode for Furazolidone detection: theoretical and experimental approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Rebelo, P (Autor) Outra; Pacheco, JG (Autor) Outra; Voroshylova, IV (Autor) FCUP; Andre Melo (Autor) FCUP; Natalia N D S Cordeiro (Autor) FCUP; Delerue Matos, C (Autor) Outra

Revista

Título: Sensors and Actuators, B: Chemical

Vol. 329

ISSN: 0925-4005

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 42 Citações

Outras Informações

ID Authenticus: P-00T-1RY

DOI: 10.1016/j.snb.2020.129112

Abstract (EN): Determination of antibiotics in environmental waters is an important global issue. Although furazolidone (FZD) was banned from use in food-producing animals, owing to its mutagenic and carcinogenic effects, this antibiotic has been illegally used across the world and its presence in environment have being noted. In this work, the first selective molecularly imprinted polymer (MIP) was developed for electrochemical detection of FZD. It was constructed based on the modification of the traditional carbon paste electrode (CPE) with MIP microparticles, followed by introduction of multi-walled carbon nanotubes (MWCNTs). Quantum mechanical (QM) calculations and molecular dynamics (MD) simulations were performed to allow rational selection of an appropriate functional monomer and to simulate the best pre-polymerisation conditions, respectively. The MIP were synthetized by polymerization using 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) as monomer and FZD as template molecule. The MIP microparticles were then incorporated on CPE-MWCNTs and the electrochemical analysis of FZD were evaluated by differential pulse voltammetry (DPV). After optimisation of experimental conditions, the MIP-CPE-MWCNTs sensor exhibited a good linear response over the concentration range of 0.01 mu M to 1 mu M with a correlation coefficient of 0.9995. The limit of detection (LOD) was found to be 0.03 mu M (S/N = 3). Due to high imprinting efficiency the sensor displayed selectivity to recognise FZD molecules and it was successfully applied in water samples where excellent recovery values (over 90 %) were obtained. The proposed sensor provides an efficient and promising sustainable strategy for monitorisation of FZD in environmental waters.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11