Disposable electrochemical immunosensor for analysis of cystatin C, a CKD biomarker

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2019

Autores

Lopes, P (Autor) Outra; Costa Rama, E (Autor) Outra; Beirao, I (Autor) Outra; Nouws, HPA (Autor) Outra; Alice Santos Silva (Autor) FFUP; Delerue Matos, C (Autor) Outra

Revista

Título: Talanta

Vol. 201

Páginas: 211-216

ISSN: 0039-9140

Editora: Elsevier

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00Q-F25

DOI: 10.1016/j.talanta.2019.04.006

Abstract (EN): Specific monitoring of cystatin C (CysC) levels in biological fluids is critical for diagnosis, treatment and mechanistic understanding of a spectrum of diseases, particularly chronic kidney disease (CKD). Despite evidences that CysC correlates with the high risk and/or progression of CKD, its use in clinical practice is still scarce. In this context, we report the development of a simple and sensitive immunosensor for the detection of CysC. The biosensor combines the technology of cost-effective screen-printed electrodes with the high specificity of a sandwich immunoassay. Optimized conditions showed that the sensor operates in a linear range between 10 and 100 ng mL(-1), with a detection limit and a sensitivity of 6.0 ng mL(-1) and 6.4 +/- 0.31 mu A ng mL(-1) cm(-2), respectively. Moreover, the sensor provided precise results (RSD <= 6.2%) and the quantification of CysC in CKD serum samples revealed to be in agreement with the values obtained by a particle-enhanced nephelometric immunoassay. In this light, the proposed immunosensor qualifies for clinical application, constituting a step forward in the development of fast, sensitive and cost-effective diagnostic tools that can improve the current medical care settings of CKD patients.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6