Disposable electrochemical immunosensor for analysis of cystatin C, a CKD biomarker

• Type: Another Publication in an International Scientific Journal
• Date: 2019

Authors

Lopes, P (Author) Other; Costa Rama, E (Author) Other; Beirao, I (Author) Other; Nouws, HPA (Author) Other; Alice Santos Silva (Author) FFUP; Delerue Matos, C (Author) Other

Journal

Title: Talanta

Vol. 201

Pages: 211-216

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-00Q-F25

DOI: 10.1016/j.talanta.2019.04.006

Abstract (EN): Specific monitoring of cystatin C (CysC) levels in biological fluids is critical for diagnosis, treatment and mechanistic understanding of a spectrum of diseases, particularly chronic kidney disease (CKD). Despite evidences that CysC correlates with the high risk and/or progression of CKD, its use in clinical practice is still scarce. In this context, we report the development of a simple and sensitive immunosensor for the detection of CysC. The biosensor combines the technology of cost-effective screen-printed electrodes with the high specificity of a sandwich immunoassay. Optimized conditions showed that the sensor operates in a linear range between 10 and 100 ng mL(-1), with a detection limit and a sensitivity of 6.0 ng mL(-1) and 6.4 +/- 0.31 mu A ng mL(-1) cm(-2), respectively. Moreover, the sensor provided precise results (RSD <= 6.2%) and the quantification of CysC in CKD serum samples revealed to be in agreement with the values obtained by a particle-enhanced nephelometric immunoassay. In this light, the proposed immunosensor qualifies for clinical application, constituting a step forward in the development of fast, sensitive and cost-effective diagnostic tools that can improve the current medical care settings of CKD patients.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6