Characterization of insulin-loaded alginate nanoparticles produced by ionotropic pre-gelation through DSC and FTIR studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Bruno Sarmento (Autor) Outra; Dorningos Ferreira (Autor) FFUP; Francisco Veiga (Autor) Outra; Antonio Ribeiro (Autor) Outra

Revista

Título: Carbohydrate Polymers

Vol. 66

Páginas: 1-7

ISSN: 0144-8617

Editora: Elsevier

Indexação

ISI Web of Knowledge - 240 Citações

Scopus - 343 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-GMS

DOI: 10.1016/j.carbpol.2006.02.008

Abstract (EN): Insulin-loaded nanoparticles were prepared by ionotropic pre-gelation of alginate with calcium chloride followed by complexation between alginate and chitosan. The influence of the pH and stoichiometry relationship between polyelectrolytes providing individual particles with a nano-scale size was assessed by photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Insulin-polyelectrolyte interactions at varying pH and polyelectrolytes stoichiometry were assessed by differential scanning calorimetry (DSC) and Fourier-transform infrared (FTIR) studies. Individual and smaller sizing nanoparticles, around 800 nm, were obtained at pH 4.7 with an alginate:chitosan mass ratio of 6:1. Thermograms of insulin-loaded nanoparticles originated shifts on same unloaded nanoparticle peaks and suggested polyelectrolytes-protein interactions at pH around 4.5-5.0. FTIR spectra of insulin-loaded nanoparticles showed amide absorption bands characteristic of protein spectra and revealed the formation of new chemical entities.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7