Characterization of insulin-loaded alginate nanoparticles produced by ionotropic pre-gelation through DSC and FTIR studies

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Bruno Sarmento (Author) Other; Dorningos Ferreira (Author) FFUP; Francisco Veiga (Author) Other; Antonio Ribeiro (Author) Other

Journal

Title: Carbohydrate Polymers

Vol. 66

Pages: 1-7

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 240 Citations

Scopus - 343 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-GMS

DOI: 10.1016/j.carbpol.2006.02.008

Abstract (EN): Insulin-loaded nanoparticles were prepared by ionotropic pre-gelation of alginate with calcium chloride followed by complexation between alginate and chitosan. The influence of the pH and stoichiometry relationship between polyelectrolytes providing individual particles with a nano-scale size was assessed by photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Insulin-polyelectrolyte interactions at varying pH and polyelectrolytes stoichiometry were assessed by differential scanning calorimetry (DSC) and Fourier-transform infrared (FTIR) studies. Individual and smaller sizing nanoparticles, around 800 nm, were obtained at pH 4.7 with an alginate:chitosan mass ratio of 6:1. Thermograms of insulin-loaded nanoparticles originated shifts on same unloaded nanoparticle peaks and suggested polyelectrolytes-protein interactions at pH around 4.5-5.0. FTIR spectra of insulin-loaded nanoparticles showed amide absorption bands characteristic of protein spectra and revealed the formation of new chemical entities.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7