The Antioxidant Peptide Salamandrin-I: First Bioactive Peptide Identified from Skin Secretion of Salamandra Genus (Salamandra salamandra)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Placido, A (Autor) Outra; Bueno, J (Autor) Outra; Barbosa, EA (Autor) Outra; Moreira, DC (Autor) Outra; Dias, JD (Autor) Outra; Cabral, WF (Autor) Outra; Albuquerque, P (Autor) Outra; Bessa, LJ (Autor) Outra; Freitas, J (Autor) Outra; Kuckelhaus, SAS (Autor) Outra; Lima, FCDA (Autor) Outra; Batagin Neto, A (Autor) Outra; Brand, GD (Autor) Outra; Relvas, JB (Autor) FMUP; Leite, JRSA (Autor) Outra; Eaton, P (Autor) Outra

Revista

Título: Biomolecules

Vol. 10

Página Final: 512

ISSN: 2218-273X

Editora: MDPI

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00R-Z9V

DOI: 10.3390/biom10040512

Abstract (EN): Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H](+) = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and -negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 mu M. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 20