The Antioxidant Peptide Salamandrin-I: First Bioactive Peptide Identified from Skin Secretion of Salamandra Genus (Salamandra salamandra)

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Placido, A (Author) Other; Bueno, J (Author) Other; Barbosa, EA (Author) Other; Moreira, DC (Author) Other; Dias, JD (Author) Other; Cabral, WF (Author) Other; Albuquerque, P (Author) Other; Bessa, LJ (Author) Other; Freitas, J (Author) Other; Kuckelhaus, SAS (Author) Other; Lima, FCDA (Author) Other; Batagin Neto, A (Author) Other; Brand, GD (Author) Other; Relvas, JB (Author) FMUP; Leite, JRSA (Author) Other; Eaton, P (Author) Other

Journal

Title: Biomolecules

Vol. 10

Final page: 512

ISSN: 2218-273X

Publisher: MDPI

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00R-Z9V

DOI: 10.3390/biom10040512

Abstract (EN): Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H](+) = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and -negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 mu M. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20