Immobilization of Human Mesenchymal Stem Cells within RGD-Grafted Alginate Microspheres and Assessment of Their Angiogenic Potential

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Silvia J Bidarra (Author) Other; Cristina C Barrias (Author) Other; Mario A Barbosa (Author) FEUP; Raquel Soares (Author) FMUP; Pedro L Granja (Author) FEUP

Journal

Title: Biomacromolecules

Vol. 11

Pages: 1956-1964

ISSN: 1525-7797

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 109 Citations

Scopus - 121 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-44S

DOI: 10.1021/bm100264a

Abstract (EN): In this work, human mesenchymal stem cells (hMSC) immobilized in RGD-coupled alginate microspheres, with a binary composition of high and low molecular weight alginate, were investigated. Cells immobilized within ROD-alginate microspheres (during 21 days) showed metabolic activity, with an overall viability higher than 90%, short cell extensions, and, when induced, they were able to differentiate into the osteogenic lineage. In osteogenic conditions (comparing to basal conditions), immobilized cells presented alkaline phosphatase (ALP) activity and an upregulation of ALP, collagen type I, and Runx 2 expression. Moreover, mineralization was also detected in immobilized cells under osteogenic stimulus. In addition, it was demonstrated for the first time that MSCs immobilized in this 3D matrix were able to enhance the ability of neighboring endothelial cells to form tubelike structures. Overall, these findings represent a step forward in the development of injectable stem cell carriers for bone tissue engineering.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9