Immobilization of Human Mesenchymal Stem Cells within RGD-Grafted Alginate Microspheres and Assessment of Their Angiogenic Potential

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Silvia J Bidarra (Autor) Outra; Cristina C Barrias (Autor) Outra; Mario A Barbosa (Autor) FEUP; Raquel Soares (Autor) FMUP; Pedro L Granja (Autor) FEUP

Revista

Título: Biomacromolecules

Vol. 11

Páginas: 1956-1964

ISSN: 1525-7797

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 109 Citações

Scopus - 121 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-44S

DOI: 10.1021/bm100264a

Abstract (EN): In this work, human mesenchymal stem cells (hMSC) immobilized in RGD-coupled alginate microspheres, with a binary composition of high and low molecular weight alginate, were investigated. Cells immobilized within ROD-alginate microspheres (during 21 days) showed metabolic activity, with an overall viability higher than 90%, short cell extensions, and, when induced, they were able to differentiate into the osteogenic lineage. In osteogenic conditions (comparing to basal conditions), immobilized cells presented alkaline phosphatase (ALP) activity and an upregulation of ALP, collagen type I, and Runx 2 expression. Moreover, mineralization was also detected in immobilized cells under osteogenic stimulus. In addition, it was demonstrated for the first time that MSCs immobilized in this 3D matrix were able to enhance the ability of neighboring endothelial cells to form tubelike structures. Overall, these findings represent a step forward in the development of injectable stem cell carriers for bone tissue engineering.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9