Relative quantification of Vitis vinifera L. varieties in musts by microsatellite DNA analysis

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Miguel Angelo Faria (Author) Other; Eugenia Nunes (Author) FCUP; Maria Beatriz P P Oliveira (Author) FFUP

Journal

Title: European Food Research and Technology

Vol. 227 No. 3

Pages: 845-850

ISSN: 1438-2377

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 18 Citations

ISI Web of Science

Scopus - 20 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-Y08

DOI: 10.1007/s00217-007-0795-5

Resumo (PT): Varietal wines are defined as made primarily from a single variety of grape, which is identified on the label. However the inclusion of other varieties in their production is permitted under defined percentages. The fact that different grape varieties/varietal wines present far different economical values can attract fraudulent practices. Thus the development of methods, which allow the quantification of those varieties, is of crucial importance. The first approach to the method for the relative quantification of must containing two varieties based on microsatellite DNA analysis is presented. Quantification was achieved by densitometry measurements of the amplification products of the pooled varieties, obtained with loci VVMD6 and VVMD7, after their separation and staining on polyacrilamide gels. We have found that a good correlation between the proportion of each variety in must and the signal intensity of the alleles are dependent on the variety (varietal effect) and the microsatellite locus used. The relative quantification of musts can be accomplished by the presented method depending on the presence of overlapping alleles, and the composition of the varieties and their relative amounts. <br> <br> Keywords: Vitis vinifera - Grape must - Microsatellite markers - Varietal authentication - Quantification <br> <a target="_blank" href="http://www.springerlink.com/content/8111252436230450/?p=1f003195d0004f69b0a0b772023e5305&pi=22"> Texto integral</a> <br> <br>

Abstract (EN): Varietal wines are defined as made primarily from a single variety of grape, which is identified on the label. However the inclusion of other varieties in their production is permitted under defined percentages. The fact that different grape varieties/varietal wines present far different economical values can attract fraudulent practices. Thus the development of methods, which allow the quantification of those varieties, is of crucial importance. The first approach to the method for the relative quantification of must containing two varieties based on microsatellite DNA analysis is presented. Quantification was achieved by densitometry measurements of the amplification products of the pooled varieties, obtained with loci VVMD6 and VVMD7, after their separation and staining on polyacrilamide gels. We have found that a good correlation between the proportion of each variety in must and the signal intensity of the alleles are dependent on the variety (varietal effect) and the microsatellite locus used. The relative quantification of musts can be accomplished by the presented method depending on the presence of overlapping alleles, and the composition of the varieties and their relative amounts.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6