Characterization of triacylglycerols in Madeira laurel oil by HPLC-atmospheric pressure chemical ionization-MS

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Paula C. Castilho (Author) Other; Maria do Céu Costa (Author) Other; Alírio Rodrigues (Author) FEUP; Pedro Costa Branco (Author) Other; Mafalda Costa (Author) Other; Ana Rodrigues (Author) Other

Journal

Title: Journal of the American Oil Chemists' Society

Vol. 81 No. 10

Pages: 913-919

ISSN: 0003-021X

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-000-8BT

DOI: 10.1007/s11746-004-1001-9

Abstract (EN): Madeira laurel oil was fractionated by liquid extraction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and by LC-atmospheric pressure chemical ionization (APCI)-MS analysis of the lipid. The APCI-MS spectra of most TAG exhibited [M + H](+) and [M - RCOO](+) ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high degree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two saturated FA (SSM or SSU, where S is saturated, M is monounsaturated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12-15degreesC) and also the high oxidative resistance observed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7