Periplasmic targeting of recombinant proteins in Escherichia coli

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Mergulhao, FJ (Author) FEUP; Monteiro, GA (Author) Other

Journal

Title: Methods in molecular biology (Clifton, N.J.)

Vol. 61

Pages: 47-61

ISSN: 1064-3745

Publisher: Springer Nature

Indexing

ISI Web of Knowledge

Scopus - 11 Citations

Other information

Authenticus ID: P-008-NTG

DOI: 10.1385/1-59745-466-4:47

Abstract (EN): Targeting recombinant protein production to the periplasmic space of Escherichia coli presents several advantages over cytoplasmic production in inclusion bodies and at the same time overcomes the low productivity problem often associated with culture medium secretion. This chapter presents a strategy for periplasmic production of recombinant proteins fused to synthetic Z domains derived from staphylococcal protein A. Expression, purification, and monitoring strategies are discussed using green fluorescent protein and human proinsulin as model proteins. © Humana Press Inc.

Language: English

Type (Professor's evaluation): Scientific