Periplasmic targeting of recombinant proteins in Escherichia coli

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Mergulhao, FJ (Autor) FEUP; Monteiro, GA (Autor) Outra

Revista

Título: Methods in molecular biology (Clifton, N.J.)

Vol. 61

Páginas: 47-61

ISSN: 1064-3745

Editora: Springer Nature

Indexação

ISI Web of Knowledge

Scopus - 11 Citações

Outras Informações

ID Authenticus: P-008-NTG

DOI: 10.1385/1-59745-466-4:47

Abstract (EN): Targeting recombinant protein production to the periplasmic space of Escherichia coli presents several advantages over cytoplasmic production in inclusion bodies and at the same time overcomes the low productivity problem often associated with culture medium secretion. This chapter presents a strategy for periplasmic production of recombinant proteins fused to synthetic Z domains derived from staphylococcal protein A. Expression, purification, and monitoring strategies are discussed using green fluorescent protein and human proinsulin as model proteins. © Humana Press Inc.

Idioma: Inglês

Tipo (Avaliação Docente): Científica