Automatic flow injection analysis (FIA) determination of total reducing capacity in serum and urine samples

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Segundo, MA (Author) FFUP; Toth, IV (Author) Other; Magalhaes, LM (Author) Other; Reis, S (Author) FFUP

Journal

Title: Methods in molecular biology (Clifton, N.J.)

Vol. 1208

Pages: 277-284

ISSN: 1064-3745

Publisher: Springer Nature

Indexing

ISI Web of Knowledge

Scopus - 1 Citation

Other information

Authenticus ID: P-00G-6VF

DOI: 10.1007/978-1-4939-1441-8_20

Abstract (EN): Automation of total antioxidant capacity assessment can substantially increase the determination throughput, allowing large scale studies and screening experiments. Total reducing capacity evaluation can be implemented under different chemistries, including the CUPRAC¿Cupric Ion Reducing Antioxidant Capacity ¿assay. This assay is based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)- neocuproine complex by reducing (antioxidant) components of biological samples. In this chapter, we propose an automatic fl ow injection method for evaluation of total reducing capacity in serum and urine samples, attaining end-point data within 4 min using a kinetic matching strategy. © Springer Science+Business Media New York 2015.

Language: English

Type (Professor's evaluation): Scientific