Automatic flow injection analysis (FIA) determination of total reducing capacity in serum and urine samples

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Segundo, MA (Autor) FFUP; Toth, IV (Autor) Outra; Magalhaes, LM (Autor) Outra; Reis, S (Autor) FFUP

Revista

Título: Methods in molecular biology (Clifton, N.J.)

Vol. 1208

Páginas: 277-284

ISSN: 1064-3745

Editora: Springer Nature

Indexação

ISI Web of Knowledge

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00G-6VF

DOI: 10.1007/978-1-4939-1441-8_20

Abstract (EN): Automation of total antioxidant capacity assessment can substantially increase the determination throughput, allowing large scale studies and screening experiments. Total reducing capacity evaluation can be implemented under different chemistries, including the CUPRAC¿Cupric Ion Reducing Antioxidant Capacity ¿assay. This assay is based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)- neocuproine complex by reducing (antioxidant) components of biological samples. In this chapter, we propose an automatic fl ow injection method for evaluation of total reducing capacity in serum and urine samples, attaining end-point data within 4 min using a kinetic matching strategy. © Springer Science+Business Media New York 2015.

Idioma: Inglês

Tipo (Avaliação Docente): Científica