Theoretical study of the efficient fluorescence quenching process of the firefly luciferin

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

da silva, lp (Autor) Outra; simkovitch, r (Autor) Outra; huppert, d (Autor) Outra; da silva, jcge (Autor) FCUP

Revista

Título: Journal of Photochemistry and Photobiology A: Chemistry

Vol. 266

Páginas: 47-54

ISSN: 1010-6030

Editora: Elsevier

Indexação

ISI Web of Knowledge - 14 Citações

ISI Web of Science

Scopus - 10 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química

Outras Informações

ID Authenticus: P-006-87A

DOI: 10.1016/j.jphotochem.2013.06.001

Abstract (EN): The firefly oxyluciferin family of fluorophores has been attracting attention from the research community, due to their role on firefly bioluminescence. Moreover, this family exhibits a very efficient fluorescence quenching process. The elucidation of this process is very important, as it may occur inside of the luciferase active site, thereby decreasing the life time of bioluminescence. To this end we have used a computational approach to study the fluorescence quenching of firefly luciferin, the most studied member of the oxyluciferin family of fluorophores. We have found that the fluorescence quenching is due to the excited state protonation of the nitrogen heteroatom of the benzothiazole moiety. This protonation leads to a singlet to triplet intersystem crossing to triplet excited states, which explains the quenching of fluorescence.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8