Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Araujo, D (Autor) Outra; Alves, VD (Autor) Outra; Lima, SAC (Autor) Outra; Salette Reis (Autor) FFUP; Freitas, F (Autor) Outra; Reis, MAM (Autor) Outra

Revista

Título: International Journal of Biological Macromolecules

Vol. 156

Páginas: 1104-1111

ISSN: 0141-8130

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00S-A6R

DOI: 10.1016/j.ijbiomac.2019.11.141

Abstract (EN): Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at -20 degrees C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterials.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8